(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of EIF4A1 expression in HT-1080 cells using EIF4A1 antibody. Green, isotype control; red, EIF4A1.

Immunocytochemical staining of HT-1080 cells with EIF4A1 antibody. Nuclei were stained blue with DAPI;EIF4A1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using EIF4A1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4A1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blot
All lanes: Eukaryotic initiation factor 4A-I antibody at 3μg/ml
Lane 1: K562 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Rat brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47, 40 kDa
Observed band size: 47 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA007550ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA007550ESR1HU at dilution of 1:100