(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ENTPD1 at 2 μg/ml can bind Anti-ENTPD1 recombinant antibody (CSB-RA007690MA1HU). The EC₅₀ is 5.294-6.760 ng/mL.

Activity
NPR1 Recombinant Monoclonal Antibody (CSB-RA007690MA1HU) captured on Protein A Chip can bind Human ENTPD1 with an affinity constant of 1.07 nM as detected by MetaSPR Assay (WeSPRTM 200).

Western Blot
Positive WB detected in: HL-60 whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate
All lanes: ENTPD1 antibody at 2.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 59, 35, 43, 47, 60 kDa
Observed band size: 58, 43 kDa

IHC image of CSB-PA007690LA01HU diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA007690LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).