(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of GALT expression in HeLa cells using GALT antibody. Green, isotype control; red, GALT.

Immunocytochemical staining of Hela cells with GALT antibody. Nuclei were stained blue with DAPI; GALT was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using GALT antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GALT antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA009226LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA009226LA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Rat liver tissue, Mouse kidney tissue
All lanes: GALT antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 32 kDa
Observed band size: 44 kDa

Immunofluorescent analysis of Hela cells using CSB-PA009226LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunoprecipitating GALT in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA009226LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA009226LA01HU (8µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)