Flow cytometric analysis of GCLC expression in HepG2 cells using GCLC antibody. Green, isotype control; red, GCLC.

Immunocytochemical staining of HepG2 cells with GCLC antibody. Nuclei were stained blue with DAPI; GCLC was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using GCLC antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GCLC antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

IHC image of CSB-PA009321LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.