(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
, Positive WB detected in:COLO205 whole cell lysate(30μg), MCF7 whole cell lysate(30μg), U87 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), A2780 whole cell lysate(30μg), Mouse brainr tissue lysate(30μg), Rat brain lysate(30μg)
All lanes: HAPLN1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 40 kDa
Observed band size: 48 kDa
Exposure time：120s

IHC image of CSB-RA179147A0HU diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing HepG2 cells stained with CSB-RA179147A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunohistochemistry of paraffin-embedded human bladder cancer using CSB-PA010130ESR2HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA010130ESR2HU at dilution of 1:100