(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Histone deacetylase 10 expression in HepG2 cells using Histone deacetylase 10 antibody. Green, isotype control; red, Histone deacetylase 10.

Immunocytochemical staining of HepG2 cells with Histone deacetylase 10 antibody. Nuclei were stained blue with DAPI; Histone deacetylase 10 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Histone deacetylase 10 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone deacetylase 10 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: Mouse liver tissue
All lanes: HDAC10 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72, 70, 71, 44 kDa
Observed band size: 72, 34 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA010236LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010236LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA010236LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of MCF-7 cells using CSB-PA010236LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)