(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 0.9519-1.213 ng/mL.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/ml can bind Human ACVR2A (CSB-MP001260HU1d9).The EC₅₀ is 74.75-84.73 ng/mL.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/ml can bind Human ACVR2B (CSB-MP623829HUi9).The EC₅₀ is 45.93-52.42 ng/mL.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/ml can bind Anti-INHBA recombinant antibody (CSB-RA011719MA1HU). The EC₅₀ is 10.28-12.68 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: Hela whole cell lysate(30μg), HepG2 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg)
All lanes: INHBA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 120s