(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Interferon regulatory factor 3 expression in HepG2 cells using Interferon regulatory factor 3 antibody. Green, isotype control; red, Interferon regulatory factor 3.

Immunocytochemical staining of HepG2 cells with Interferon regulatory factor 3 antibody. Nuclei were stained blue with DAPI; Interferon regulatory factor 3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using Interferon regulatory factor 3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Interferon regulatory factor 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blot analysis of hela Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200