(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Immunofluorescence staining of A549 with CSB-RA788545A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 519-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA788545A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western blot
All lanes: KRT6A antibody at 4µg/ml
Lane 1: A431 whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA012561LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA012561LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA012561LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)