(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: COLO205 whole cell lysate(30μg)
All lanes: LCK antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 2min

Overlay Peak curve showing jurkat cells stained with CSB-RA583933A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA012806LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA012806LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA012806LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunoprecipitating LCK in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA012806LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA012806LA01HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)