Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody. Green, isotype control; red, LDL receptor related protein 8.

Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody. Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using LDL receptor related protein 8 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LDL receptor related protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: SY5Y whole cell lysate(30μg), THP-1 whole cell lysate(30μg), U251 whole cell lysate(30μg)
All lanes: LRP8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 106,78,100,88 kDa
Observed band size: 106 kDa
Exposure time: 120s