(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015691HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFM.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015691HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFM.

Flow cytometric analysis of Neurofilament medium chain expression in Hela cells using Neurofilament medium chain antibody. Green, isotype control; red, Neurofilament medium chain.

Immunocytochemical staining of Hela cells with Neurofilament medium chain antibody. Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Neurofilament medium chain antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neurofilament medium chain antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

The image is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA680148(NEFM Antibody) at dilution 1/20. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Human brain malignant glioma tissue, Primary antibody: CSB-PA680148(NEFM Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minutes