(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Phospho-PP2A alpha (Y307) expression in HAP-1 cells using Phospho-PP2A alpha (Y307) antibody. Green, isotype control; red, Phospho-PP2A alpha (Y307).

Immunocytochemical staining of HAP-1 cells with Phospho-PP2A alpha (Y307)?antibody. Nuclei were stained blue with DAPI; Phospho-PP2A alpha (Y307)? was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using Phospho-PP2A alpha (Y307) antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PP2A alpha (Y307) antibody and HRP-conjugated goat rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
All lanes: PPP2CA antibody at 2.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 30 kDa
Observed band size: 36, 30 kDa

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA018559LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA018559LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)