(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of Protein kinase C eta expression in HepG2 cells using Protein kinase C eta antibody. Green, isotype control; red, Protein kinase C eta.

Immunocytochemical staining of HepG2 cells with Protein kinase C eta antibody. Nuclei were stained blue with DAPI; Protein kinase C eta was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Protein kinase C eta antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Protein kinase C eta antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: HEK293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue, Rat kideny tissue, Rat stomach tissue, Mouse heart tissue, Mouse kidney tissue, Mouse stomach tissue
All lanes: PRKCH antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 78, 60 kDa
Observed band size: 78 kDa

IHC image of CSB-PA15879A0Rb diluted at 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA15879A0Rb diluted at 1:700 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.