(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019259HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RAD23A.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019259HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RAD23A.

Flow cytometric analysis of RAD23A expression in HepG2 cells using RAD23A antibody. Green, isotype control; red, RAD23A.

Immunocytochemical staining of HepG2 cells with RAD23A antibody. Nuclei were stained blue with DAPI; RAD23A was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human lymphoma using RAD23A antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using RAD23A antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD23A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blot
All lanes: RAD23A antibody at 3µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Raji whole cell lysate
Lane 4: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40, 34 kDa
Observed band size: 34 kDa

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA03774A0Rb at dilution of 1:100