(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: COLO205 whole cell lysate(30μg)
All lanes: RAD51 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3min

IHC image of CSB-RA251963A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela cell with CSB-RA251963A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing jurkat cells stained with CSB-RA251963A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate
All lanes: RAD51 antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 27, 32 kDa
Observed band size: 37 kDa

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA019268LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA019268LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunoprecipitating RAD51 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1μg) instead of CSB-PA019268LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA019268LA01HU (4μg) + K562 whole cell lysate (500μg)
Lane 3: K562 whole cell lysate (20μg)