(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg), HepG2 whole cell lysate(30μg),MCF-7 whole cell lysate(30μg), PC-3 whole cell lysate(30μg), Hela whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), U-251MG whole cell lysate(30μg)
All lanes: SDHA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 73 kDa
Observed band size: 65 kDa
Exposure time：1min

IHC image of CSB-RA620235A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA620235A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing Hela cells stained with CSB-RA620235A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western blot
All lanes: SDHA antibody at 10µg/ml
Lane 1: Rat heart tissue
Lane 2: Mouse spleen tissue
Lane 3: Mouse liver tissue
Lane 4: Hela whole cell lysate
Lane 5: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 73, 68, 57 kDa
Observed band size: 73 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA01989A0Rb at dilution of 1:50