Western Blot
Positive WB detected in: A431 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), BxPC-3 whole cell lysate(30μg), PC-3 whole cell lysate(30μg), Hela whole cell lysate(30μg), A549 whole cell lysate(30μg),MCF7 whole cell lysate(30μg),Mouse Brain tissue lysate(30μg)
All lanes: SLC38A2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 57,46 kDa
Observed band size: 57 kDa
Exposure time: 120s

Immunofluorescence staining of A431 cell with CSB-PA847210LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A431 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).