(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of SMN1 expression in HAP-1 cells using SMN1 antibody. Green, isotype control; red, SMN1.

Immunocytochemical staining of H9c2 cells with SMN1 antibody. Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using SMN1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMN1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate
All lanes: SMN1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32, 29, 31, 28 kDa
Observed band size: 40, 60 kDa

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA614982LA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA614982LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)