(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: Rat brain tissue, Mouse brain tissue
All lanes: STXBP1 antibody at 2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68, 69 kDa
Observed band size: 68, 69 kDa

Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA022905LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA022905LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Flow cytometric analysis of syntaxin binding protein 1 expression in C2C12 cells using syntaxin binding protein 1 antibody. Green, isotype control; red, syntaxin binding protein 1.

Immunocytochemical staining of C2C12 cells with Syntaxin binding protein 1 antibody. Nuclei were stained blue with DAPI; Syntaxin binding protein 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using syntaxin binding protein 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with syntaxin binding protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.