(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of TAF15 expression in HepG2 cells using TAF15 antibody. Green, isotype control; red, TAF15.

Immunocytochemical staining of HepG2 cells with TAF15 antibody. Nuclei were stained blue with DAPI; TAF15 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using TAF15 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using TAF15 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAF15 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA954760(TAF15 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA954760(TAF15 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Jurkat cells, hepg2 cells, Raji cells, A431 cells, Primary antibody: CSB-PA954760(TAF15 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds