(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western blot
All lanes: Nucleolysin TIA-1 isoform p40 antibody at 2μg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43, 42, 25 kDa
Observed band size: 43 kDa

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA023527DSR1HU at dilution of 1: 100

Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA023527DSR1HU at dilution of 1: 100

Immunoprecipitating TIA1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA023527DSR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA023527DSR1HU (4μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Flow cytometric analysis of TIA1 expression in HAP-1 cells using TIA1 antibody. Green, isotype control; red, TIA1.

Immunocytochemical staining of HAP1 cells with TIA1 antibody. Nuclei were stained blue with DAPI; TIA1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using TIA1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TIA1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.