(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western blot
All lanes: WNT4 antibody at 8μg/ml + MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40, 33 kDa
Observed band size: 40 kDa

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA026137LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA026137LA01HU at dilution of 1:100

IHC image of CSB-PA026137LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.