(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Flow cytometric analysis of XRCC3 expression in HeLa cells using XRCC3 antibody. Green, isotype control; red, XRCC3.

Immunocytochemical staining of Hela cells with XRCC3 antibody. Nuclei were stained blue with DAPI; XRCC3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-XRCC3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-XRCC3 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.

Western Blot analysis of HepG2 cells using XRCC3 Polyclonal Antibody