Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody. Green, isotype control; red, 14-3-3 alpha/beta.

Immunocytochemical staining of HepG2 cells with 14-3-3 alpha/beta antibody. Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using 14-3-3 alpha/beta antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 14-3-3 alpha/beta antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate, Rat brain tissue, Rat kidney tissue, Mouse kidney tissue
All lanes: YWHAB antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29, 28 kDa
Observed band size: 29 kDa

IHC image of CSB-PA01544A0Rb diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA01544A0Rb diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA01544A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).