Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Mouse liver tissue
All lanes: CBS antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 62 kDa
Observed band size: 61 kDa
Immunohistochemistry analysis of human glioma using CSB-PA12719A0Rb at dilution of 1:100
Immunocytochemistry analysis of human pancreatic tissue using CSB-PA12719A0Rb at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA12719A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating CBS in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA12719A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA12719A0Rb (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期:
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
相關疾病:
Cystathionine beta-synthase deficiency (CBSD)
亞細胞定位:
Cytoplasm. Nucleus.
蛋白家族:
Cysteine synthase/cystathionine beta-synthase family
組織特異性:
In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.