(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP004589HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CBS.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP004589HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CBS.

Western Blot
Positive WB detected in: MCF7 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), HEK293 whole cell lysate(30μg)
All lanes:CBS antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time：2min

IHC image of CSB-RA242578A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA242578A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA242578A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Mouse liver tissue
All lanes: CBS antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 62 kDa
Observed band size: 61 kDa

Immunohistochemistry analysis of human glioma using CSB-PA12719A0Rb at dilution of 1:100

Immunocytochemistry analysis of human pancreatic tissue using CSB-PA12719A0Rb at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA12719A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunoprecipitating CBS in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA12719A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA12719A0Rb (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)